Effect of antiasthma simplified herbal medicine intervention (ASHMI) on bronchoalveolar lavage fluid (BALF) total cell counts (A-D) and eosinophil numbers (E-H). After airway response measurement, mice were humanely killed, and the lungs were lavaged. Total leukocytes were counted, and BALF was cytocentrifuged on slides, fixed, and stained for leukocyte differential. A-D, Mean BALF total cell counts in nontreated ovalbumin (OVA) mice, ASHMI-treated OVA mice, and naive mice aged 6 weeks (A), 6 months (B), 12 months (C), and 18 months (D). E-H, Mean BALF absolute eosinophil counts in the same mouse groups aged 6 weeks (E), 6 months (F), 12 months (G), and 18 months (H). Error bars represent SEM. *P<.05, **P<.01, ***P<.001 compared with age-matched nontreated OVA mice.