Table 1. Primer sequences and amplification summary.
| Gene* | Reference | 5'-3' sequence | Amplicon lenght (pb) | PCR efficiency (%) |
|---|---|---|---|---|
| Clock | AB019258.1 | F-CTTCAGTTCAGCAGCCAGC | 125 | 109,00 |
| R-GCTCTGTTGTAGTGGAAAGGCA | ||||
| Cry1 | NM_198750.2 | F-CAGTTGGGAAGAAGGGATGAAG | 60 | 99,78 |
| R-ATGCTCCAGTCGGCGTCAA | ||||
| Per1 | NM_001034125.1 | F-GCAGAAACAACAGCCACGGT | 115 | 103,93 |
| R-GTCCACACAAGCCGTTACATCG | ||||
| Per3 | NM_023978.2 | F-CCACAGCATCAGTACAGCAAG | 142 | 90,22 |
| R-GCTCTGTCTCTCTGTCTATCCT | ||||
| Bma1l | NM_024362.2 | F-CCGTGGACCAAGGAAGTAGA | 97 | 97,66 |
| R-CTGTGAGCTGTGGGAAGGTT | ||||
| Cry2 | NM_133405.2 | F-ATTGAGCGGATGAAGCAGAT | 103 | 98,63 |
| R-CCACAGGGTGACTGAGGTCT |
*Clock, Circadian locomotor output cycle kaput; Cry1-2, Cryptochrome1-2; Per1-3, Period 1–3; Bmal1, Brain and muscle Arnt-like protein-1.