Table 2. Primers used for quantitative RT-PCR investigation.
Primer & probes | Sequence |
---|---|
p4b-For-qPCR | 5’-AGTGAAACAAAGTGGGGCCATC-3’ |
p4b-Rev-qPCR | 5’-GAAATGCATCCCTCCTGTTACGG-3’ |
p4b taqman probe | 5’-CTATTGCTCTCGGGCCAAATGCGGACC-3’ |
EF-1a-For-qPCR | 5’-AGAACATGATTACAGGCACTTCCC-3’ |
EF-1a-Rev-qPCR | 5’-GGAAATACCTGCTTCGAATTCACC-3’ |
EF-1a taqman probe | 5’-ACCAGCAGCAACAATCAGGACAGCACA-3’ |
p4b primer & probes: Amplicon length100 bp. Protocol: 95C / 3 min, 95C / 10 sec, 61,4C / 30 sec, 40 cycles
EF-1a primer & probes: Amplicon length 86 bp. Protocol: 95C / 3 min, 95C / 10 sec, 61.4C / 30 sec, 40 cycles