Table 4. Intensity ratios of FVIII to VWF measured along EC-secreted VWF strings.

	String	FVIII	VWF	FVIII to VWF
	Lengths, μm (String #s)	Mean Intensity	Mean Intensity	Intensity Ratio (range)
GMVECs 60×	8–51 (45)	78.4 ± 35.2	83 ± 41.9	1.13 ± 0.6 a (0.23–2.7)
GMVECs 100×	4–27 (23)	79.6 ± 46.6	61.7 ± 33.3	1.4 ± 0.6 b (0.47–3.1)
HUVECs 60×	15–84 (15)	59.8 ± 60.2	91.4 ± 27.7	0.67 ± 0.6 (0.17–2.3)
HUVECs 100×	17–51 (14)	40.6 ± 16	75.5 ± 20.6	0.6 ± 0.3 (0.24–1.3)

Histamine-stimulated GMVECs and HUVECs were stained with rabbit anti-human VWF plus chicken anti-rabbit IgG AF-488, followed by staining with mouse FVIII plus goat anti-mouse IgG AF-647. Intensity values from the 488-nm and the 647-nm channels were measured along merged images of the EC-secreted and anchored ULVWF strings. Data (means ± SD) were collected from 4 HUVEC and 11 GMVEC experiments.

^aIndicates P = 0.01 compared to HUVEC values at 60×

^bIndicates P = <0.001 compared to HUVEC values at 100×