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. 2015 Oct 16;10(10):e0140745. doi: 10.1371/journal.pone.0140745

Fig 4. miR-101 expression in prostate cancer cells.

Fig 4

A, Expressions of pri-miR-101 and mature miR-101 were determined in AR positive or negative cell lines by qPCR. **, P<0.01 between two compared groups. B, LNCaP or 22Rv1 cells were transfected with AR siRNA or control siRNA (ctrl siRNA). AR knockdown effects were verified by Western blotting. Expressions of pri-miR-101 and mature miR-101 were determined by qPCR (C). *, P<0.05 versus control siRNA. D, DU145 or PC-3 cells were transfected with pEGFP-C1-AR (EGFP-AR) or empty vector (EGFP-V) and treated with DMSO or celastrol (CEL, 2 μM) for 24 h. Expressions of mature miR-101 were determined by qPCR. *, P<0.05 between EGFP-AR and EGFP-V transfections. E, LNCaP cells were treated with R1881 (1 nM) for 24 h after androgen starvation, as described in Fig 2 C. AR protein levels were determined by Western blotting using GAPDH as a loading control. MiR-101 expressions were determined by qPCR. **, p<0.01 versus DMSO.