Fig 3. Representative histopathology of spleen and brain of live-virus vaccinated C57bl/6 mice post-challenge.
Mice previously infected with 105 PFU of CHIK or chimeric viruses (ΔDomA, ΔDomB or ΔDomC) were challenged with 105 PFU of SFV. Surviving mice were euthanized and spleens and brains were harvested followed by processing for Hematoxylin and Eosin (H&E) staining. A-F. Spleens at 10x magnification. The scale bars represent 100 μM. G-L. Hippocampal neurons at 25x magnification. The scale bar represents 50 μM. Arrows signify neuron degeneration.