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. 2015 Oct 16;9(10):e0004163. doi: 10.1371/journal.pntd.0004163

Fig 6. Neutralization of parental and chimeric viruses with CHIKV-immune human serum.

Fig 6

Convalescent serum samples from 10 human patients were collected aseptically. Neutralization was performed using plaque reduction neutralization 50 (PRNT50) test. Two-fold dilutions of serum samples were incubated individually with each parental (CHIKV or SFV) or chimeric virus (ΔDomA, ΔDomB or ΔDomC, ΔDomA+B). Cells were fixed 36 hours post-infection and stained with crystal violet solution to visualize plaques. Data is presented as the inverse of the final serum dilution that showed 50% reduction or greater in plaques.