Fig 1. Acer3 is upregulated with age in the mouse brain.
A. mRNA levels of the alkaline ceramidases Acer1, Acer2, and Acer3 in the cerebra and cerebella of young [6 weeks (6W) of age] versus middle-aged mice [8 months (8M) of age] Note that the mRNA levels of Acer3 but not Acer1 or Acer2 were increased in mice at 8M of age compared to at 6W. B. Acer3 mRNA levels in the cerebellum versus the cerebrum of 6W or 8M-old mice. Note that Acer3 mRNA levels were higher in the cerebellum than in the cerebrum in the same mouse at either 6W or 8M of age. C. Alkaline ceramidase activity on NBD-C12-PHC in the brains of young (6W) versus middle-aged mice (8M). Note that the alkaline ceramidase activity was higher in both cerebrum and cerebellum in 8M-old-mice compared to 6W-old mice and that the alkaline ceramidase activity was higher in the cerebellum than in the cerebrum in mice at either age. Data in A, B, and C represent mean values ± SD, n = 3.