Table 2. Mutant Allele Frequency measurement accuracy.

Quantification uncertainty varies inversely with single nucleotide variant MAF, with the highest relative uncertainty at lower MAF and least percent relative uncertainty at higher MAF.

MAF range (%)	Quantification Uncertainty % (Absolute Error)
> 10	4% (> 0.4)
3–10	6% (0.18–0.6)
1–3	14% (0.14–0.42)
0.5–1.0	20% (0.1–0.2)
0.25–0.5	30% (0.075–0.15)