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. 2015 Oct 16;10(10):e0140312. doi: 10.1371/journal.pone.0140312

Table 3. Detection of HCV in Two Sequential Paired Plasma and PBMC.

First Sample Collections Second Sample Collections
HCV RNA + Strand HCV RNA + Strand
Plasma Plasma PBMC
Patient Age/Sex Genotype 250 μl ~5 ml PBMC PHA-Treated HCV RNA-Strand PBMC Time from First Collection (Years) 250 μl ~5 ml PHA-Treated CS-Treated HCV RNA-Strand PBMC
4-6/M 1b NEG NEG NEG NT 5.2 NEG POS POS NT NEG
7-73/F NT NEG NEG NEG NT 5.5 NEG NEG NEG NEG NT
10-70/M 1a NEG NEG POS NEG 6.5 NEG NEG NEG NEG NT
14-47/F 2a NEG POS POS NEG 4.5 NEG NEG NEG POS POS
15-46/F 3a POS NT POS POS 5.1 NEG POS NEG POS POS
16-52/F 1b NEG NEG POS NEG 7.2 POS NT NEG NEG NT
17-47/M NT NEG NEG NEG NT 6.3 NEG NEG NEG NEG NT
20-49/F 1b POS NT NEG NT 6.3 NEG POS POS NT POS
24-53/M 1b NEG POS NA NA 6.7 NEG NEG NEG POS POS
Mean or Number From Total 2/9 2/7 4/8 1/4 5/9 1/9 3/8 2/9 3/7 4/5

PBMC, peripheral blood mononuclear cells; HCV RNA+, HCV RNA positive strand; HCV RNA-, HCV RNA negative strand; PHA, phytohemagglutinin; C5, immune cell stimulating cocktail (see Materials and Methods); POS, positive; NEG, negative; NT, not tested; NA, not available.