Table 3. Detection of HCV in Two Sequential Paired Plasma and PBMC.
First Sample Collections | Second Sample Collections | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
HCV RNA + Strand | HCV RNA + Strand | ||||||||||
Plasma | Plasma | PBMC | |||||||||
Patient Age/Sex | Genotype | 250 μl | ~5 ml | PBMC PHA-Treated | HCV RNA-Strand PBMC | Time from First Collection (Years) | 250 μl | ~5 ml | PHA-Treated | CS-Treated | HCV RNA-Strand PBMC |
4-6/M | 1b | NEG | NEG | NEG | NT | 5.2 | NEG | POS | POS | NT | NEG |
7-73/F | NT | NEG | NEG | NEG | NT | 5.5 | NEG | NEG | NEG | NEG | NT |
10-70/M | 1a | NEG | NEG | POS | NEG | 6.5 | NEG | NEG | NEG | NEG | NT |
14-47/F | 2a | NEG | POS | POS | NEG | 4.5 | NEG | NEG | NEG | POS | POS |
15-46/F | 3a | POS | NT | POS | POS | 5.1 | NEG | POS | NEG | POS | POS |
16-52/F | 1b | NEG | NEG | POS | NEG | 7.2 | POS | NT | NEG | NEG | NT |
17-47/M | NT | NEG | NEG | NEG | NT | 6.3 | NEG | NEG | NEG | NEG | NT |
20-49/F | 1b | POS | NT | NEG | NT | 6.3 | NEG | POS | POS | NT | POS |
24-53/M | 1b | NEG | POS | NA | NA | 6.7 | NEG | NEG | NEG | POS | POS |
Mean or Number From Total | 2/9 | 2/7 | 4/8 | 1/4 | 5/9 | 1/9 | 3/8 | 2/9 | 3/7 | 4/5 |
PBMC, peripheral blood mononuclear cells; HCV RNA+, HCV RNA positive strand; HCV RNA-, HCV RNA negative strand; PHA, phytohemagglutinin; C5, immune cell stimulating cocktail (see Materials and Methods); POS, positive; NEG, negative; NT, not tested; NA, not available.