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Primer sequences used in RT-PCR
| Name | Sequence (5′–3′) | Ta (°C) |
|---|---|---|
| SPTLC1 (F) | AAGAAGCCATTATATACTCATAT | 58 |
| SPTLC1 (R) | GGCACTGATAAGATCAATA | 58 |
| SPTLC2 (F) | GAGTGTGTACAACAGTTAGCTG | 58 |
| SPTLC2 (R) | TGGCT CACAAAGGCCAC | 58 |
| GAPDH (F) | AGG TCG GAG TCA ACG GAT TTG | 53 |
| GAPDH (R) | ATG GGT GGA ATC ATA TTG GAA CAT G | 53 |
F, forward; R, reverse.
