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. 2015 Jul 14;29(11):4532–4543. doi: 10.1096/fj.15-276493

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© The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — NCX1 is palmitoylated in heart, brain and kidney. A) Palmitoylated proteins were purified from homogenates of heart, brain, and kidney and immunoblotted as shown. Note the extended incubation at 40°C during acyl-RAC causes partial aggregation of NCX1 resulting in 2 species of ∼120 and 150 kDa resolved by SDS-PAGE. B) Fraction of NCX1.1 palmitoylated in ventricular muscle. A small fraction of NCX1.1 is not palmitoylated in ventricular muscle. NCX1.1 enrichment in acyl-RAC reactions was normalized to the constitutively palmitoylated protein caveolin 3 (Cav3). n = 5, mean ± sem shown. C) NCX1.1 is palmitoylated in human-induced pluripotent stem cells differentiated into cardiac myocytes. P, proteins purified by acyl-RAC; Palm, proteins purified using acyl-RAC in either the presence (+) or absence (−) of hydroxylamine (ha); UF, unfractionated cell lysate; UB, material not captured by acyl-RAC.