TABLE 1.
Compositions of solutions in electrophysiology experiments
| Ingredient |
Fig. 4A |
Fig. 4B |
Fig. 5A |
Figs. 5B and 7 |
Fig. 6 |
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|---|---|---|---|---|---|---|---|---|---|---|
| Pipette | Bath | Pipette | Bath | Pipette | Bath | Pipette | Bath | Pipette | Bath | |
| NaOH (mM) | 40 | 70 | 0 | 120 or 0 | 40 | 70 | 40 | 0 | 40 | 120 |
| LiOH (mM) | 0 | 0 | 0 | 0 or 120 | 0 | 0 | 0 | 0 | 0 | 0 |
| NMDG (mM) | 85 | 70 | 120 | 0 | 85 | 70 | 85 | 120 | 85 | 0 |
| TEA-OH (mM) | 15 | 10 | 15 | 10 | 15 | 10 | 15 | 20 | 15 | 15 |
| HEPES (mM) | 15 | 10 | 15 | 10 | 15 | 10 | 15 | 10 | 15 | 10 |
| EGTA (mM) | 20 | 0.5 | 20 | 0.5 | 20 | 0.5 | 0.5 or 10 | 0.5 | 10 | 0.5 |
| CaCO3 (mM) | 2 or 10 | 0 | 10 or 18 | 0 | 0 | 0 | 0 | 0 | 0 | |
| CaCl2 (mM) | 0 | 0 or 4 | 0 | 0 | 0 | 0 or 4 | 0.25 or 1 | 0 or 4 | 1 | 0 or 4 |
| MgCl2 (mM) | 0.5 | 4 or 0 | 0.25 | 4 | 0.25 | 4 or 0 | 0.5 | 4 or 0 | 0.5 | 4 |
| MgATP (mM) | 8 | 0 | 8 | 0 | 8 | 0 | 0 | 0 | 0 | 0 |
| TrisATP (mM) | 2 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
| Asp to pH (mM) | 7.0 | 7.4 | 7.0 | 7.4 | 7.0 | 7.4 | 7.0 | 7.4 | 7.0 | 7.4 |
| AlCl3 (mM) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.15 | 0 |
| Free pipette Ca (µM) | 0.1 or 0.5 | 0.5 or 3.0 | 0 | 0.5 or 0.1 | 0.1 | |||||