FIG. 1.

Schematic illustration of the experimental procedure performed on the integrated microfluidic chip for screening of cancer cell-specific peptides, including positive selection, negative selection, and phage amplification steps. (a) Negative selection using magnetic bead-coated control cells and a phage display library. (b) Removal of unwanted phages by applying a magnet and transporting the phages of interest to the positive selection chamber. (c) Positive selection using magnetic bead-coated target cells and the phage display library from the step (b). (d) Collection of phages of interest by using a magnet and the washing of un-bound or weakly bound phages. (e) Amplification of phage binding on target cells by propagating on E. coli.