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. 2015 Oct 16;15:215. doi: 10.1186/s12866-015-0557-7

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© Faria et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Enzymatic digestion requirement for bacterial DNA extraction of whole blood. Whole blood was spiked with a CBA plate pool from ASN087 BAL sample. Following a treatment with saponin, the resulting pellet was either digested with no enzyme (black), lysozyme (grey), or lysozyme and mutanolysin (blue). More T-RFs were present when enzymatic digestion was done with lysozyme (10, grey) and lysozyme plus mutanolysin (24, blue) identified, respectively, when compared to no enzymatic treatment with only 7 T-RFs present