Table 3.

Percent recovery and limit of detection for mock community spiked into whole blood

	Percent recovery (%)									Limit of detection (CFU/ml)
	Saponin alone			Saponin + 1 Wash			Saponin + 2 washes			Saponin alone	Saponin +1 wash	Saponin + 2 washes
Organism	SC1	SC3	SC5	SC1	SC3	SC5	SC1	SC3	SC5
E. coli	82.8	100	73.7	24.8	0	0	32.3	51.7	15.5	102	104	103
E. hormaechei	100	100	100	100	100	100	100	100	100	10	10	10
N. flava	100	100	23.4	100	100	75.6	100	100	37.8	105	104	104
M. luteus	100	68.7	8.0	100	6.9	0	20.1	0	0	102	10	10
S. epidermidis	100	100	100	100	100	39.6	100	100	100	10	10	10
S. aureus	100	100	39.3	100	100	31.7	100	100	100	10	10	10
S. intermedius	100	100	100	100	100	19.9	100	100	100	10	10	10
S. pneumoniae	100	100	0	96.9	100	0	100	100	100	102	102	10
F. necrophorum a	0	100	0	0	0	0	0	0	0	102	103	104
P. melaninogenica a	100	100	0	100	0	0	0	0	0	102	102	102

^aBased on recovery alone (Fig. 3) not in SC