Fig. 6.

GEM-treated MIA PaCa-2 cells in G2M showed higher HER2 expression than those in S or G1. a MIA PaCa-2 cells were treated with GEM (0 or 300 ng/ml) for 2 h, washed with PBS and incubated for 96 h. The cells were examined for cell cycle status by flow cytometry. M1: G1 phase, M2: S phase, M3: G2M phase. b HER2 expression in G1, S and G2M cell populations of untreated or GEM-treated MIA PaCa-2 cells. The cell population in each phase was gated, and HER2 expression was examined by flow cytometry. HER2 (ΔMFI) was calculated as the MFI of PE-labeled anti-human HER2 minus that of the isotype control. The experiments were repeated three times with similar results, and a representative result is shown