Table 2.

Thermodynamic properties for parkin activation

Protein	Activator	Kd (μM)	N	ΔH (kJ/mol)	ΔS (J/molK)
A. R0RBR Parkin with Ubl and Ub
R0RBR	Ubl	3.8 ± 1.3	0.98	−44 ± 3	−44
	Ubl S65D	5.0 ± 0.9	1.03	−38 ± 1	−29
	Ubl S65E	5.3 ± 1.2	0.98	−46 ± 2	−54
	pUbl	26 ± 10	1.04	−16 ± 9	33
R0RBR	Ub	67 ± 1.0	1.05	31 ± 1	184
	Ub S65D	6.6 ± 0.3	1.01	25 ± 1	184
	Ub S65E	6.6 ± 0.1	1.10	48	261
	pUb	0.016 ± 0.002	0.93	32	261
B. Full-length Parkin with Ubl and Ub
Parkin	Ubl	N.O.	–	–	–
	Ub	45 ± 9	0.90	10 ± 1	115
	pUb	0.16 ± 0.02	1.09	50	293
		0.37 ± 0.04a	–	–	–
pParkin	pUb	0.021 ± 0.006
		0.017 ± 0.005a	–	–	–

Average values from a minimum of two experiments.

^aData from Ordureau et al (2014).

N.O.,binding not observed.