M–O Single Lgr5-GFP stem cells were isolated by FACS from vehicle (
n = 4) or DBZ-treated (
n = 4) mice. See
Appendix Fig S4 for FACS gating scheme. (O) Organoid establishment efficiency was quantified 3 days post-plating. *
P = 0.014 versus vehicle using Student's
t-test. Data are expressed as mean ± SE.