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. 2015 Aug 12;34(20):2522–2536. doi: 10.15252/embj.201490583

Figure EV4.

Figure EV4

Chronic Notch activation in LGR5+ stem cells decreases Wnt signaling
  • A–C qRT–PCR analysis for Wnt target genes Lgr5 (A) and Axin2 (B) in control (n = 7) and Lgr5; ROSANICD (n = 7) mice 1 year post-TX, or Lgr5 (C) in control, DAPT-treated and Lgr5; ROSANICD antral organoids (n = 8 per group) (mean ± SE). *P < 0.05 and ***P < 0.001 vs. −NICD using Student's t-test. **P < 0.01 vs. Control using one-way ANOVA.
  • D, E GFP immunostaining in control and Lgr5; ROSANICD mice 1 year post-TX. NICD activation showed lack of cytoplasmic Lgr5-GFP expression in hyperplastic polyps. Arrows indicate cytoplasmic Lgr5-GFP+ stem cells at the gland base. (E′) Prominent nuclear GFP resulting from NICD activation in Lgr5; ROSANICD antral polyp. Scale bars: 50 μm.
  • F, G Lgr5 re-tracing with the ROSACon reporter in Lgr5; ROSANICD mice. Re-tracing was observed in non-polyp tissue (F; arrowhead), but not in a polyp region (G). Scale bars: 50 μm.
  • H–J Lgr5 in situ hybridization in control and Lgr5; ROSANICD mice 1 year post-TX. Lgr5 expression (J; arrow) was markedly reduced in polyp areas. Scale bars: 50 μm.