Skip to main content
. Author manuscript; available in PMC: 2016 Oct 16.
Published in final edited form as: ACS Synth Biol. 2015 Jun 2;4(10):1077–1085. doi: 10.1021/acssynbio.5b00053

Figure 2. Bem1 sequestration with DeLight prevents cells from budding.

Figure 2

(a) bem1Δ and Bem1 DeLight strains (+PCB, Bem1-sequestered or −PCB, not sequestered) were pre-treated with infrared light and then switched to red light to activate sequestration at t=0 and imaged in DIC for a period of 10 hours; see Movie 2. (b) Cell diameter for each condition, measured every five minutes (error bars = standard error of mean, SEM; 10 cells measured for each condition at each timepoint). (c) Average cell diameters at the beginning and end of the experiment (error bars = SEM, 10 cells measured for each condition at each timepoint). (d) Schematic of phenotypes for cell scoring. (e) Quantification of movies from (b). Cells were scored for phenotypes based on the schematic in (d). (f) Quantification of cell lysis during the 10-hour red light exposure. For panels (e) and (f), >100 cells were scored for each condition. Acute inhibition of Bem1 with DeLight produces more profound defects in budding and cell viability than genetic nulls of Bem1.