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. Author manuscript; available in PMC: 2016 Oct 15.
Published in final edited form as: Clin Cancer Res. 2015 Jun 12;21(20):4698–4708. doi: 10.1158/1078-0432.CCR-15-0157

Figure 6.

Figure 6

SRRM4 expression and REST splicing in patient metastases and LuCaP xenografts. (A) PCR demonstrating REST is spliced inserting a 62 bp exon producing REST4 (indicated by arrow) in AR− and AR+ metastatic sites from each of four patients. LuCaP 145.1 (neuroendocrine, splicing control); LuCaP 73 (adenocarcinoma, no splicing control). (B) Western analysis of SRRM4 in adenocarcinoma and neuronal xenografts, (C) PCR demonstrating REST is spliced producing REST4 (indicated by arrow) in the 4 neuronal LuCaP PDX lines, and (D) qPCR of REST, SRRM4, and SNAP25 (a gene repressed by REST) expression with actin as a reference in neuronal and adenocarcinoma LuCaP PDX lines. Transcript levels are defined relative to LuCaP 96 (an adenocarcinoma).

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