TABLE 1.

Ponatinib, AZD4547 and AZD8055 sensitivity of lung and HNSCC cell lines

Cell Line	Tissue and histology	Ponatinib IC50 (nM)	AZD4547 IC50 (nM)	AZD8055 IC50 (nM)	AZD2014 IC50 (nM)
H520	Lung squamous cell carcinoma	53	57	23	21
Colo699	Lung adenocarcinoma	14	6	61	4
H1703	Lung adenosquamous carcinoma	24	460	60	8
CCL30	Nasopharynx, squamous cell carcinoma	ND	9	66	ND
H1581	Lung large cell carcinoma	1	2	78	40
H1299	Lung large cell carcinoma	140	>1000	178	25
H157	Lung squamous cell carcinoma	119	>1000	632	210
584-A2	Larynx, squamous cell carcinoma	ND	6	>1000	ND

The tissue source and histology of the cancer cell lines used in the studies is summarized. The sensitivity of the cell lines to ponatinib is from (2). The sensitivities of the cell lines to the FGFR inhibitor, AZD4547, and the MTOR inhibitor, AZD8055, were determined with clonogenic or anchorage-independent growth assays using drug concentrations from 0 – 1 μM. The sensitivity of the cell lines to AZD2014 was measured using cell proliferation assays and the CyQUANT reagent over drug concentrations from 0 – 1 μM. The primary data for AZD8055 sensitivity on six of the cell lines are shown in Figure 2C and the IC₅₀ values were calculated with the Prism software program.