Skip to main content
. Author manuscript; available in PMC: 2016 Oct 15.
Published in final edited form as: Cancer Res. 2015 Aug 6;75(20):4384–4397. doi: 10.1158/0008-5472.CAN-15-0457

Figure 3. miR-221-222 targets PUMA expression through binding to the 3’UTR region of mRNA.

Figure 3

(A) Q-RT-PCR analysis of miR-221 (top) and miR-222 (middle) expression as well as reporter activity (bottom) in MM1S cells mock-transfected (cel-miR-67) or transfected with miR-221-222. Wild-type (wt), but not mutant (mut), pmiR-PUMA 3′UTR reporter activity was inhibited by miR-221-222. * p<0.05. (B) Q-RT-PCR analysis of miR-221 (top) and miR-222 (middle) expression as well as reporter activity (bottom) in MM1R cells mock-transfected (scrambled mRNA) or transfected with 2’-OMe as-miR-221-222. Wild-type, but not mutant, pmiR-PUMA 3′UTR reporter activity was increased by knockdown of miR-221-222. * p<0.05.