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. Author manuscript; available in PMC: 2016 Oct 15.
Published in final edited form as: Cancer Res. 2015 Aug 6;75(20):4384–4397. doi: 10.1158/0008-5472.CAN-15-0457

Figure 4. Enhanced miR-221-222 expression decreases PUMA expression in MM1S cells in vitro.

Figure 4

Q-RT-PCR analysis of miR-221 (A) and miR-222 (B) expression in MM1S cells lentivirally transduced with empty vector (V-GFP) or V-miR-221-222-GFP. ** p<0.01. Analysis of PUMA expression at the mRNA (C) and protein (D) level in MM1S cells lentivirally transduced with empty vector (V-GFP) or with V-miR-221-222-GFP. * p<0.05. (E) Immunoblot analysis of PARP and caspase 3 activation in MM1S cells lentivirally transduced with empty vector (V-GFP) or with V-miR-221-222-GFP, in the presence or absence of Dex. (F) Viability assay of MM1S cells lentivirally transduced with empty vector (V-GFP) or with V-miR-221-222-GFP after treatment with Dex. ** p<0.01. (G) Immunocytochemical analysis of cleaved caspase-3 expression in MM1S cells lentivirally transduced with empty vector (V-GFP) or with V-miR-221-222V-GFP, then treated with Dex or left untreated.