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. Author manuscript; available in PMC: 2016 Oct 15.
Published in final edited form as: Cancer Res. 2015 Aug 17;75(20):4272–4282. doi: 10.1158/0008-5472.CAN-14-3319

Figure 1.

Figure 1

Derivation and characterization of the ZMEL1 zebrafish melanoma cell line. a) Representative melanoma bearing fish (left) from the MiniCoopr background which mosaically expresses BRAFV600E in a mitfa-GFP;p53−/− background, and yielded the stable cell line ZMEL1, which is uniformly GFP positive. b) Growth curves of the ZMEL1 line demonstrate a population doubling time of 1.6 days. Individual colors representative replicate experiments. c,d) The response of the ZMEL1 and ZMEL-R1 lines to either the BRAFV600E inhibitor PLX4032 (c) or the MEK inhibitor CI1040 (d). The resistant line demonstrates an 8-fold increase in the EC50 to the BRAF inhibitor, but only a 2-fold increase in the EC50 to the MEK inhibitor.