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. Author manuscript; available in PMC: 2016 Oct 15.
Published in final edited form as: Mol Cell. 2015 Oct 1;60(2):268–279. doi: 10.1016/j.molcel.2015.08.023

Figure 6. mec1-100 suppresses defects of cells lacking Rtt107 or its associated E3s.

Figure 6

(A) mec1-100 de-represses late origins in rtt107Δ cells. Replication fork-associated ssDNA profiles of a segment of Chromosome XV are shown as in Figure 5A.

(B) 2D gel analyses confirm that mec1-100 leads to firing of late origins, ARS1212 and ARS1413, in rtt107Δ background. Experimental procedures and labels are identical to those in Figure 5B.

(C) mec1-100 increases chromosomal replication in rtt107Δ cells. Experimental scheme and results are presented as in Figure 5C and S5B. Quantification of results from 3 trials is plotted with means and SD.

(D) mec1-100 rescues the MMS sensitivity of rtt107Δ and related E3 mutants.

(E) Side-by-side comparison shows that mec1-100 is less effective than mrc1Δ in suppressing the MMS sensitivity of rtt107Δ cells. Experiments were done as in Figure 5E, except that plates were grown for a shorter time to better reveal differences between mec1-100 and mrc1Δ suppression.

See also Figure S6.