Figure 6.

Chicken chorioallantoic membrane in vivo analysis. Autoclaved filter paper punches with conditioned medium from scaffolds after 48 h in culture were observed over a five-day period for neovascularization of the CAM. Note the increase in small vessel convergence from the scaffold, specifically in VEGF, collagen-GAG, and fibrin matrices (a). Samples exposed to conditioned medium without cells are not pictured, as they did not differ from the negative control (PBS). Growth was analyzed from 6 replicates per treatment based on an arbitrary scoring system dealing with new small vessel formation and the behavior of existing vessels as observed daily according to [40]. Briefly, a value was assigned for each 5 d ranging from (0) unchanged to slight changes in density and convergence towards filter paper punch (1) and further increases in density and convergence (up to 5) (b). 5 mm scale bar. ^∗ p < 0.05, ^∗∗∗ p < 0.001, ^∗∗∗ p < 0.0001 when compared to VEGF positive control.