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. 2015 Oct 4;2015:148367. doi: 10.1155/2015/148367

Figure 2.

Figure 2

TRPA1 is important for the induction of IL-8 by CSE in HBECs. (a) Cells were incubated with medium alone or exposed to 3% CSE for 24 hours and pretreated with 3–9 μM HC-030031 (HC, a TRPA1 antagonist) or its vehicle DMSO. (b) Cells were incubated with or without two concentrations (25 and 50 nM) of TRPA1 siRNA (siTRPA1) for 24 hours prior to the measurement of TRPA1 expression. (c) Cells were incubated with medium alone or exposed to 3% CSE for 24 hours having been pretreated with 50 nM of either siRNA (siTRPA1) or scramble siRNA. Data in each group are mean ± SEM from five independent experiments. ∗ p < 0.05 versus the control or versus time zero; # p < 0.05 versus CSE alone.