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. 2015 Oct 4;2015:148367. doi: 10.1155/2015/148367

Figure 7.

Figure 7

Comparisons of cigarette smoke- (CS-) induced oxidative stress and lung inflammatory score in wild-type and trpa1 −/− mice. Mice were chronically exposed to air or CS for 4 weeks. (a) Levels of ROS in the bronchoalveolar lavage fluid (BALF) were sampled immediately after the last air or CS exposure across the four study groups. Levels of ROS were measured by DCFH-DA fluorescent probe assay. (b) Expression of 4-HNE modified protein, a biomarker of oxidative stress, in lung tissues was analyzed by Western blotting. (c) Representative images of H&E stained lung sections obtained from air-exposure or CS-exposure wild-type and trpa1 −/− mice. (d) Lung inflammatory scores were calculated according to the sum of the levels of cell infiltration and damage levels assessed from the lung sections. Data in each group are mean ± SEM from nine mice. ∗ p < 0.05 versus the air-exposure group in both genotypes; # p < 0.05 versus the CS-exposure wild-type group.