Mice were vaccinated SL with the indicated dose of either flu antigen alone or flu antigen in combination with MGC, CRX-601 or MGC-CRX-601. Anti-flu IgA titers in vaginal wash samples (VW, black circles) and tracheal wash samples (TW, grey squares) collected on day 56 (14dp3 for SL and 14dp2 for IM) are shown. The horizontal dashed line represents the LLOQ for the VW (black line, ~54.02 ng/mL) and TW (grey line, ~62.18 ng/mL) ELISA assays. Values equal to or less than the LLOQ at 1:40 sample dilution are represented as a value of 1. The number of mice per group that had titers greater than LLOQ, out of the total mice per group, is shown on the X-axis (some TW samples were not assayed due to contamination with blood). The mean and standard error are indicated. Data were analyzed by one-way ANOVA with Dunnett post-test. *, p <0.005, **, p≤0.001, ***, p <0.0001 compared to the flu antigen only group.