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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Acta Biomater. 2015 Aug 28;27:194–204. doi: 10.1016/j.actbio.2015.08.039

Figure 8.

Figure 8

(a) HPLC analysis of nanogels degradation products to confirm the recovery of pure quercetin and not any other mono or higher quercetin acrylates. This was confirmed by comparing the nanogel degradation products with quercetin, QMA (monomer) and degradation products of bulk Q-PBAE gels which results in quercetin and quercetin monoacrylate release, (b) Degradation/quercetin release profile from Q-PBAE nanogels in PBS (pH 7.4 with 2vol% DMSO). 90% of the quercetin was released within 35 hours of incubation. N=3 and the profile fits Korsmeyer-Peppas release kinetic model Mt/Minf = Ktn with n values within 0.45–0.85 showing anomalous release. R-square ranged from 0.9737 to 0.9923, (c) Antioxidant activity profile of the degraded products of Q-PBAE nanogels. The study was done using the trolox equivalent antioxidant capacity assay (TEAC), and the activity is expressed in terms of equivalent amount of trolox. (n=3, M±SD) (d) Instantaneous active quercetin values calculated from TEAC values compared with instantaneous quercetin released values obtained after each degradation time interval.