TABLE 1.
Primers used in this study
| Primer | Sequence (5′ to 3′) | Source or reference |
|---|---|---|
| FP | GTGATGGGGTGCAAGCTCTT | This study |
| RP | TCTGGCGACTCGAGTTCTGC | 7 |
| MPa | ATCTACCCGCGGCTAGACAGG | This study |
| WPa | ATCTACCCGCGGCTAGACAGA | This study |
a
The primers MP and WP contained a specific mismatch A (underlined) at the 3′end that did not complement the published sequence of 23S rRNA of B. pertussis (see Fig. 1). The G (boldfaced) at the 3′ end of the MP primer was complementary to 2047G of erythromycin-resistant B. pertussis, whereas the A (boldfaced) at the 3′ end of the WP primer was complementary to A2047 of erythromycin-sensitive B. pertussis.