TABLE 2.
Comparison of the results from PCR-based sequencing and the allele-specific PCR assay in determination of the A2047G mutation of 23S rRNA in B. pertussis from 200 nasopharyngeal swab samples submitted for diagnostic PCRa
| Allele-specific PCR assay | PCR sequencing assay |
Total | ||
|---|---|---|---|---|
| Wild type | Mutation type | Negative | ||
| Wild type | 14b | 0 | 0 | 14 |
| Mutation type | 0 | 81c | 0 | 81 |
| Negative | 1 | 4 | 100 | 105 |
| Total | 15 | 85 | 100 | 200 |
a
The 200 nasopharyngeal (NP) swab samples included 100 positive and 100 negative samples tested by diagnostic ptx-Pr PCR (7). Of the 100 ptx-Pr PCR positive samples, 16 were also culture positive. Of the 16 B. pertussis strains, 14 were erythromycin resistant and 2 were erythromycin sensitive.
b
Containing the 2 NP samples from which B. pertussis was isolated and proven to be erythromycin sensitive.
c
Containing the 14 NP samples from which B. pertussis was isolated and proven to be erythromycin resistant.