FIG 1.
Melting curve peaks for the full-length erm(41) gene at 85.8°C (median) for 69/90 (77%) of isolates and truncated erm(41) gene at 78.4°C (median) for 21/90 (23%) isolates. The assay was run in triplicates. Isolates with a median Tm of 85.8°C include CI12-1, CI12-8, CI12-9, CI12-13, CI12-14, CI12-15, CI12-16, CI12-19, CI12-20, CI4-1, CI6-7, CI6-5, CI6-4, and CI6-3, M. abscessus ATCC 19977T, and M. bolletii 501898T. The isolates with a median Tm of 78.4°C are CI12-10, CI12-2, and M. massiliense 48898T.