TABLE 1.

Primers used in the full and truncated erm(41) gene discrimination assay, single nucleotide polymorphism, and sequencing of the erm(41) and 23S rRNA rrl genes of the M. abscessus group

Primer	Sequence	Purpose	Gene ID no.	Product size (bp)
erm225 Forward	5′-GGCACATCTGGTTGCCGC-3′	SYBR green erm(41) real-time assay	MAB_2297 M. abscessus	321 or 47
erm546Rev_RC Reverse	5′-CGGCAATGTGATTCCGGCC-3′
MycoSNP_Fex Forward	5′-GAGCATGGGCATATTCATGATGG-3′	erm(41) position 28 SNP real-time assay	MAB_2297 M. abscessus	125
MycoSNP_Rex Reverse	5′-TGAGCGAACACCGGATTCG-3′
23S_SNPF Forward	5′-GCGAAATTGCACTACGAGTAAAG-3′	23S rRNA position 2058/2059 SNP real-time assay	MAB_5052 M. abscessus	110
23S_SNPR Reverse	5′-CCTATCCTACACAAACCGAACC-3′
ermF Forward	5′-GACCGGGGCCTTCTTCGTGAT-3′	erm(41) sequencing	MAB_2297 M. abscessus	672 or 396
ermR1 Reverse	5′-GACTTCCCCGCACCGATTCC-3′
23S_18 Forward	5′-AGTCGGGACCTAAGGCGAG-3′	23S rRNA sequencing	MAB_5052 M. abscessus	1,525
23S_21 Reverse	5′-TTCCCGCTTAGATGCTTTCAG-3′
23SrRNAF_207 Forward	5′-AGCGAAATTCCTTGTCGGGT-3′	23S rRNA sequencing	MAB_5052 M. abscessus	207
23SrRNAR_207 Reverse	5′-CTGCTTCACAGTCTCCCACC-3′