FIG 2.

Hdac3 is required for progression through early thymocyte development. (A) FACS analysis of GFP⁺ double-negative (CD4⁻/CD8⁻) thymocytes using CD44 and CD25 to delineate DN1 to DN4. The numbers in the lower corners of the gates indicate the percentages of cells analyzed. (B) FACS analysis of intracellular TCRβ expression by DN3 and DN4 cells. (C) (Left) FACS analysis of total thymocytes using CD4 and CD8 markers, with the results for CD8⁺ cells being replotted using CD147 and CD3 expression. (Right) Bar graph presenting the composite results for 6 mice.