FIG 7.

PPM1G is required for NF-κB-mediated Pol II transcription elongation at the A20 gene in response to etoposide. (A) PPM1G KD abolishes A20 gene expression in response to etoposide. The cell lines from Fig. 6A (HeLa NTsh and PPM1Gsh) were used for total RNA extraction, and A20 gene expression in response to etoposide was quantified by qRT-PCR (means ± standard errors of the means; n = 3). The data were normalized to levels of the β-actin transcript. (B) ChIP assay of cell lines from Fig. 6A showing levels of NF-κB, PPM1G, Pol II, and 7SK snRNP components (Cdk9, Hexim1, and Larp7) at the A20 promoter-proximal region (−27 amplicon) and gene body (+17751 amplicon) in response to a time course of etoposide treatment (means ± standard errors of the means; n = 3). Normal serum (IgG) was used as a negative control to demonstrate the specificity of enrichment with the antibodies used.