FIG 5.

Ezh2 and menin binding profiles at genes showing examples of cooccupancy in ES or T cells. Binding of Ezh2, Dpy30, and menin and modifications of histone H3K27me3 and H3K4me3 at representative loci in ES cells (pink) and T cells (green) are shown. ChIP-Seq profiles are shown across six loci (chromosome 10, 111650000 to 111750000 [A]; chromosome 5, 46000000 to 46050000 [B]; chromosome 1, 120900000 to 121000000 [C]; chromosome 4, 6873211 to 6950000 [D]; chromosome 2, 9750000 to 9850000 [E]; chromosome 7, 99850000 to 99950000 [F]). Ezh2Ref (GSE23943), Dpy30 (GSE26136), and histone modification (GSE23943) data sets were obtained from the GEO database. For the visualization of binding, data sets from GSE23943 underwent the same data processing as the data sets of the present study, as described in Materials and Methods. The data sets from GSE26136 were used without data processing. The Gata3 gene showed a low UD index for Ezh2 and a high UD index for menin in T cells (Ezh2, 0.109; menin, 0.501) and was highly transcribed (E) (Fig. 4B). The binding region of Ezh2 and menin around the Gata3 TSS region does not overlap, an observation consistent with our previous findings (14). The Rab30 gene was expressed at low levels, and Ezh2 bound mainly downstream of the TSS with menin binding mainly upstream of the TSS (F) (Fig. 4B).