FIG 3.

The relative importance of AMPK subunits on colon cancer survival. (A) Viability of HCT116 cells under anchorage-independent conditions was assessed following RNAi of KSR1 or AMPKγ1. (B) Validation of individual siRNA duplexes targeting AMPKγ1. (C) Immunoblotting and apoptosis in HCECs and HCT116 cells following RNAi of AMPKγ1. (D to F) Immunoblotting and apoptosis in HCT116 cells following RNAi of AMPKα1α2 (D), AMPKα1 or AMPKα2 (E), and AMPKβ1, AMPKβ2, or AMPKβ1β2 (F). ****, P < 0.0001. RLU, relative light units.