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. 2015 Oct 16;35(22):3866–3879. doi: 10.1128/MCB.00528-15

FIG 5.

FIG 5

AMPK is activated in colon cancer cells and regulates PGC1β and ERRα in an ERK-independent manner. (A) Immunoblots of PGC1β, ERRα, phospho- and total MEK1/2, and phospho- and total ERK1/2 in HCT116 cells following RNAi of AMPKγ1. (B) Immunoblot of PGC1β and ERRα protein expression following siRNA-mediated depletion of AMPKγ1 in SW480 and SW620 cell lines. (C) Immunoblot of PGC1β and ERRα protein expression following siRNA-mediated depletion of AMPKα1 or AMPKα2 in HCT116 cells. (D) Phosphorylation of AMPK at threonine 172 and AMPKα protein expression in HCT116 cells following K-Ras, KSR1, or AMPKγ1 depletion. (E) Phosphorylation of AMPK at threonine 172 in a panel of colon tumor cell lines.