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. 2015 Aug 25;309(8):E715–E726. doi: 10.1152/ajpendo.00153.2015

Fig. 1.

Fig. 1.

IL-1β reciprocally regulates chemokine and insulin secretion. A: 832/13 cells were stimulated with 1 ng/ml IL-1β for the indicated times. Secretion of insulin (solid line) or CCL2 (dashed line) into the culture media was quantified by ELISA. B: 832/13 cells were stimulated with 1 ng/ml IL-1β for the indicated times. Relative mRNA abundance of inducible nitric oxide synthase (iNOS) was quantified by RT-PCR, with protein abundance determined by immunoblotting (inset). C: 832/13 were treated with 1 ng/ml IL-1β for the indicated times, and nitrite accumulation in the culture media was assessed using the Griess assay. D: 832/13 and 833/15 insulinoma cells were either untreated (control) or stimulated with both 1 ng/ml IL-1β and 100 U/ml IFNγ for 18 h. Insulin secretion was measured in response to basal (2 mM) or stimulatory (16 mM) glucose concentrations. E: isolated rat islets were treated with AdCMV-β-galactosidase (β-Gal) or AdCMV-IκBα super-repressor (IκBαSR) in the presence or absence of 1 ng/ml IL-1β and 100 U/ml IFNγ. Insulin secretion during static incubation was measured and represented as percent of the stimulated control. ***P < 0.001 vs. untreated (NT; black bars). Data are presented as means ± SE of 3 independent experiments. **P < 0.01; *P < 0.05. NS, not significant.