Fig. 5.

Nitric oxide decreases oxygen consumption rate without affecting mitochondrial number. A–D: 832/13 and 833/15 cells were NT or treated with 1 ng/ml IL-1β alone or IL-1β + 100 U/mL IFNγ for either 6 or 18 h. Mitochondrial number was determined using the ratio of mitochondrial DNA (CO1 or ATP6) relative to nuclear DNA (NDUFA or SDHA). E and F: 832/13 cells were exposed to 1 ng/ml IL-1β in the presence or absence of 1 mM l-NMMA for 18 h. Cellular ability to reduce the MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt] dye (E) and area under the curve (AUC) analysis of oxygen consumption rate (OCR; F) are shown. Values represent means ± SE of 3 independent experiments. **P < 0.01; *P < 0.05.