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. 2015 Aug 25;309(8):E715–E726. doi: 10.1152/ajpendo.00153.2015

Fig. 7.

Fig. 7.

NF-κB, but not glucose or nitric oxide concentration, controls production of the chemokines CCL2 and CCL20 in response to IL-1β. A and B: 832/13 cells were cotreated for 6 h with 1 ng/ml IL-1β and 1 mM l-NMMA. C and D: 832/13 cells were transduced with adenoviruses overexpressing either AdCMV-β-Gal or increasing doses of AdCMV-IκBαSR (low and high). Twelve hours after adenoviral transduction, cells were then NT or exposed to 1 ng/ml IL-1β for an additional 6 h. E and F: 832/13 cells were NT or exposed to 1 ng/ml IL-1β for 6 h in the presence of increasing concentrations of glucose (2, 4, 8, or 16 mM). Release of CCL2 (A, C, and E) and CCL20 (B, D, and F) into the culture media was measured by ELISA assays. Data represent means ± SE of a minimum of 3 independent experiments. ***P < 0.001 vs. β-Gal-IL-1β; **P < 0.01 vs. β-Gal-IL-1β.