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. 2015 Sep 1;309(8):E759–E766. doi: 10.1152/ajpendo.00346.2015

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Fig. 2. — A: omission of peptide YY (PYY) primary antibody (negative control) resulted in no PYY fiber immunofluorescence in the lPBN. B: PYY immunohistochemistry confirmed the presence of PYY fibers (white arrow) in the lPBN. C–E: representative ×20 magnification image of a Gi section: (C) green immunofluorescence represents PYY, (D) red immunofluorescence represents lPBN-injected Fluorogold(FG), (E) merged image shows colocalization of FG and PYY (white arrow). F: representative image of lPBN FG injection.