Skip to main content
. 2015 Aug 19;309(8):F697–F707. doi: 10.1152/ajprenal.00294.2015

Table 1.

Compositions of solutions (mM)

KCl NaCl NaMeSO3 CaCl2 CaGluc MgCl2 MgGluc HEPES BAPTA Dibromo-BAPTA d-Glucose
Cytoplasmic
    0.1–0.3 μM Free Ca2+ 140 5 0.7–1.2 2 5 2 5
    3 μM Free Ca2+ 140 5 1.4 2 5 2 5
    Unbuffered Ca2+ 140 5 0.03–10 2 5 5
    High-Na+ (Ca2+unbuffered) 145 1 2 5 5
    High-K+ (Ca2+unbuffered) 145 0.1–1 2 5 5
    Cl-free (Ca2+unbuffered) 145 1 2 5 5
NaCl KCl CaCl2 MgCl2 HEPES NaPyr d-Glucose
External
    Standard 140 5 2 2 5 2 9.4
    High-K+ 145 2 2 5 2 9.4
    High-Ca2+ 100 2 5 9.4

For each reagent, total concentrations are shown. NaMeSO3, sodium methanesulfonate; CaGluc, calcium gluconate; MgGluc, magnesium gluconate; NaPyr, sodium pyruvate. Total and free Ca2+ were the same in the unbuffered high-Ca2+ solutions. BAPTA and dibromo-BAPTA were added as the tetrapotassium salts. Solutions were adjusted to pH 7.4 with NaOH (high-Na+ solutions), KOH (high-K+ solutions), or Tris base (high-Ca2+ external solution).