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Summary of primers used to generate aquaporin-2 (AQP2) C-terminal phosphorylation mutants and sequence of generated AQP2 mutants. A: list of primers used to generate wild-type AQP2 and AQP2 all A/G mutant. B: comparison of the amino acid sequence of AQP2 C terminus between human and rodents. C: list of primer pairs used for site-directed mutagenesis to generate phosphorylation mimics.
