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. 2015 Oct 19;5:15160. doi: 10.1038/srep15160

Figure 4. The extent of the IONPs internalization by CMI is proportional to the initial IONPs concentration and the centrifugation time.

Figure 4

(A) Optical microscopy images of U251 cells after Prussian blue staining following IONPs internalization by CMI at 1000 rpm and different IONPs concentrations (10, 25 and 50 μg/ml) and Centrifugal Force application times (1, 5, 10 and 15 minutes). Scale bar: 40 μm and 10 μm; (B) Labeling efficiency of U251 with IONPs internalized by CMI in all different sceneries. (C) Intracellular iron content per U251 cell measured by ICP as a function of centrifugation time for an initial Fe concentration of 50 μg/ml. Error bars represent the standard deviation ***P < 0.001.