Fig. 5.

LDHA is required for radiation-driven myofibroblast differentiation. Primary human lung fibroblasts were transfected with either an LDHA siRNA pool or a nontargeting control siRNA pool 18 h before exposure to 7 Gy irradiation. A and B: cell lysates were collected 5 days postradiation and were analyzed for LDHA and α-SMA protein expression levels by Western blot and densitometry. (n = 3). C: TGF-β bioactivity was measured in cell supernatants using a mink lung cell bioassay (n = 6). D: ECAR was measured using a Seahorse Bioscience XF96 Flux Analyzer (n = 20–22). *P ≤ 0.05 by ANOVA.