Fig. 4.

ASC is not required for pulmonary IL-1β production in response to an acute PA14 infection. C57BL/6 (n = 7) and ASC^−/− (n = 7) mice were infected intratracheally with a sublethal dose (3 × 10⁶ CFU) of WT PA14. C57BL/6 mice instilled with PBS (n = 5) were used as a control. Mice were euthanized 4 h postinfection and BAL samples were collected. A: representative dot plot quantifying Ly6G⁺ and CD11c⁺ cells within a CD45⁺pro-IL-1β⁺ gate that excludes bacteria at 4 h postinfection. B: percentage of pro-IL-1β⁺Ly6G⁺ (left) and pro-IL-1β⁺CD11c⁺ (right) cells out of the total BAL cells at 4 h postinfection. C: percentage of Ly6G⁺CD11c⁻ (left) and Ly6G⁻CD11c⁺ (right) within the gated CD45⁺pro-IL-1β⁺ population as depicted in A. Data are expressed as means ± SD from 4 independent biological experiments.